2022 Conference Schedule (Tentative)

Last updated on 23.09.2022
The information contained herein is subject to change without prior notice. We will keep updating programs in the coming days, please check back again.
Day 1: Tuesday, 18 October 2022
TIME TOPIC PRESENTER
10:30 Opening remarks
10:40 Clinical-grade preservation of Advanced Therapy Medicinal ProductsINSTITUTE OF EXPERIMENTAL MEDICINE OF THE CAS, INSTITUTE OF PHYSIOLOGY OF THE CAS, BIOCEV / CZECH REPUBLIC
11:00 Aidpath - modular approach to ai-enabled car-t cell manufacturingFRAUNHOFER INSTITUTE FOR PRODUCTION TECHNOLOGY / GERMANY
11:20 The modulation of cellular hcn4 channel trafficking in hipsc-derived pacemaker-like cells during enteroviral infection with coxsackievirus b3WWU MÜNSTER / GERMANY
11:40 Automated manufacturing of 3d in vitro test systemsFRAUNHOFER ISC / GERMANY
12:00 Break
13:30 Novel single cell sequencing technologiesSINGLERON BIOTECHNOLOGIES / GERMANY
14:00 Animal-free and chemically defined coating solution for perfect 3d cell culturesFACELLITATE GMBH / GERMANY
14:25 Break
15:30 Hetero-spheroids for the study of the cross-talk between tumor cells and cafs in different metastatic cancer modelsUNIVERSITY OF MILAN-BICOCCA - DEPARTMENT OF BIOTECHNOLOGY AND BIOSCIENCES / ITALIA
15:55 Development of a 3d cell model of skeletal muscle cells (myospheres) from human primary satellite cells.UNIVERSITY OF OSLO / NORWAY
Reserved

17:00 End
Day 2: Wednesday, 19 October 2022
TIME TOPIC PRESENTER
09:30 Opening remarks  
09:40 Metabolic-imaging of human glioblastoma explants: a new precision-medicine model to predict tumor treatment response early FONDAZIONE PISANA PER LA SCIENZA / ITALIA
10:00 One-pot formation and functional monitoring of neural cysts and organoids EINDHOVEN UNIVERSITY OF TECHNOLOGY / NETHERLANDS
10:20 Cellbox - the shipment solution for living cells, organoids and sensitive probes. CELLBOX SOLUTIONS / GERMANY
10:50 O2 and ph measurements in live spheroids and cell culture PRESENS PRECISION SENSING GMBH / GERMANY
11:20 Break
13:00 The Blue®Plate; a novel microtitre plate for gentle non-contact washing of organoids via centrifugal evacuation. BLUECATBIO GMBH / GERMANY
13:30 Non-invasive pet-imaging of car-t cell therapy using dtpa-r, a novel reporter gene system TECHNICAL UNIVERSITY OF MUNICH / GERMANY
14:10 A PIK3CA-mutant breast cancer metastatic patient-derived organoid approach to evaluate alpelisib treatment for multiple secondary lesions UCBM / ITALY
Reserved







16:00 End

SPEAKING PROPOSAL 2022

The listing of a proposal does not imply an approval of a presentation. The final programme will be released here closer to the event.
The information contained herein is subject to change without prior notice.

One-pot formation and functional monitoring of neural cysts and organoids
Eindhoven University of Technology
Netherlands

Three-dimensional (3D) cell spheroids and organoids have presented a new paradigm for studying the fundamental processes behind organ and tissue regeneration, as well as for developing treatments for diseases, in physiologically relevant 3D tissue contexts. However, robust and reproducible generation of spheroids and organoids remain challenging. In this talk, we will discuss several bioengineering approaches that we have developed to obtain neuroepithelial and neural organoids from human induced pluripotent stem cells in a controlled and tunable manner. The engineering-based approaches allow longitudinal monitoring and characterization, and are also shown to be particularly suitable for basic morphogenesis studies and disease modeling of neurodegeneration.

Hetero-spheroids for the study of the cross-talk between tumor cells and CAFs in different metastatic cancer models
University of Milan-Bicocca - Department of Biotechnology and Biosciences
ITALY

Desmoplastic tumors are highly aggressive and difficult to eradicate due to the complex interactions between epithelial cells and microenvironment, which play a key role in stimulating pro-survival and progression programs. The cross-talk between cancer-associated fibroblasts (CAFs) and tumor cells, in particular, is responsible for the high invasivity of these tumors and resistance to chemotherapy. Hetero-spheroids obtained by co-culturing fibroblasts and epithelial cells of pancreatic and metastatic breast cancer were developed to determine the molecular and functional changes obtained upon different cell types interaction. The role of some soluble mediators (TGFβ and IL-6) in the cross-talk CAFs-tumor cells has been investigated. Identification of involved mediators and pathways is of great importance for the identification of new therapies against these malignant tumors.

Non-invasive PET-imaging of CAR-T Cell Therapy using DTPA-R, a Novel Reporter Gene System
Technical University of Munich
GERMANY

We developed a novel reporter gene system for non-invasive tracking of Cell-, Viro- and Gene-therapies using Positron Emission Tomography (PET), which consists of a membrane-anchored Anticalin binding protein (DTPA-R) and a corresponding radiohybrid radioligand [18F]F-DTPA. The simple design of the reporter protein with a minimal gene size yields high receptor densities of up to ~1x106 receptors per T cell and highly specific binding of the corresponding radioligand [18F]F-DTPA was proven in preclinical proof-of-principle studies for CAR-T cell therapy. In this studies we quantified proliferation and migration of anti-CD19 CAR-T cells over one month of therapy.

A PIK3CA-mutant breast cancer metastatic patient-derived organoid approach to evaluate alpelisib treatment for multiple secondary lesions
UCBM
ITALY

Breast cancer (BC) is a powerful example of the intra- and interpatient heterogeneity of tumours; thus, there remain several grey areas in BC treatment approaches. This is especially true for advanced/metastatic disease (mBC). Organoid cultures are suitable models for studying the histological complexity and genetic heterogeneity of parental tumours and can be used to assess treatment strategies for challenging diseases such as mBC. Here, we report a proof-of-concept test case using organoid cultures from metastatic BC specimens to rapidly test drug sensitivity and molecular-lesion-driven treatments. Based on the relevant impact of PIK3CA gene mutations on breast cancer progression and resistance to therapy, we assessed the efficacy of alpelisib, a specific PI3K-α inhibitor, on organoid cultures derived from multisite mBC samples carrying specific PIK3CA gene mutations.

Novel single cell sequencing technologies
Singleron Biotechnologies
GERMANY

High throughput single cell sequencing is enabled by Singleron's cutting-edge technology which allows you to study genes and their functions across tens of thousands of cells simultaneously in a single experiment. It offers you essential insights into the samples with high level of heterogeneity and provide you with crucial information on gene expression across a multitude of cell subtypes present in your sample.The applications that go beyond gene expression profiling are gaining on popularity as well, as they enable you to obtain deeper insights on the function of specific genes by adding additional layers of information, such as for instance temporal resolution, to your data.

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Cellbox - the shipment solution for living cells, organoids and sensitive probes.
Cellbox Solutions
GERMANY

The trend to use complex human-like assay systems for drug discovery and drug profiling continues to increase. Also, therapeutic modalities like cells, tissues and mini organs are increasingly applied to patients. All these probes must not be frozen but need to be shipped to collaborators or patients at distinct conditions. The Cellbox offers a temperature, gas and pH controlled mobile incubator to ship such probes around the world. We will present the Cellbox in various application of such demand.

Automated manufacturing of 3D In Vitro test systems
Fraunhofer ISC
GERMANY

The Translational Centre for Regenerative Therapies (TLC-RT) of Fraunhofer ISC has a strong experience on the design, development and validation of human 3D tissue models with applications in basic and preclinical research. Our models are based on human primary cells, 3D organoid culture or different stem cell types such as human induced pluripotent stem cells (iPSCs) grown on suitable bioscaffolds. Recreation of innate physiological conditions is addressed by tissue culture in bioreactor platforms. Also, we are developing new technologies such as noninvasive assessment methods or automated tissue production to improve the quality and availability of the tissues. We will demonstrate two lead application in the automated manufacturing i.e. human 3D skin models as well as organoids.

The modulation of cellular HCN4 channel trafficking in hiPSC-derived pacemaker-like cells during enteroviral infection with Coxsackievirus B3
WWU Münster
GERMANY

The SafeLegs is a supportive lower-limb 6-DOF exoskeleton demonstrator equipped with safety features. It is the result of collaboration between Uni Stuttgart and KIT. It consists of six actuators - brushless motors in the hips, knees, and ankles. Sensor signals are providing feedback from the motor encoders to the main system controller via the CAN bus. The SafeLegs is a complex Cyber-Physical System with components that prone to heterogeneous faults. To ensure safety we proposed a Deep Learning-based approach for system failure prevention. We employed LSTM network for error detection and mitigation. Trained LSTM models were quantized and deployed on an Edge TPU. The light weight of the system and minimal power consumption allowed its integration into wearable robotic systems.

Metabolic-imaging of human glioblastoma explants: a new precision-medicine model to predict tumor treatment response early
Fondazione Pisana per la Scienza
ITALY

FLIM-based metabolic imaging is used on live glioblastoma 3D explants to test anti-neoplastic drugs. Unlike other models, drug response of live patient-derived tumor explants is performed with no animal involvement and with minimal tampering of natural tumor cyto-architecture, preventing the conventional structural and molecular divergence that the tumor undergoes after long time of in vitro culturing. This novel in vitro approach could be exploited in a range of clinical and laboratory settings to improve patients' clinical management. A method to assess the patient’s tumor specific response to several therapies at an early stage could be transformative for glioblastoma patients’ management. Furthermore, this model can be used for large-scale screening of new pharmaceutical compounds and applied to other cancers.

O2 and pH measurements in live spheroids and cell culture
PreSens Precision Sensing GmbH
GERMANY

Spheroids are a powerful tool to study effects of drugs. The oxygenation status within spheroids and their different zones (proliferation, quiescence, necrotic zone), is of essential interest for drug development. The hypoxia can lead to e.g. quiescent or necrotic cores. The oxygenation status can be a measure for the efficacy of anticancer drugs and can help to study and choose suitable and personalized drugs or concentrations. Information on time and spatially resolved hypoxia in live 3D cell cultures still lacks proper, biocompatible, and non-destructive techniques. We present a label-free technique for spatio-temporally resolved monitoring in spheroids under growth conditions over longer periods of time. It enables automated, non-invasive measurement inside a normal incubation system and provides 2D-cross-section O2-information.

The Blue®Plate; a novel microtitre plate for gentle non-contact washing of organoids via centrifugal evacuation.
BlueCatBio GmbH
GERMANY

Blue®Plate allows for automated, gentle and non-contact washing of 3D cultures via centrifugal evacuation in the Blue®Washer. The Blue®Plate is an SBS formatted 96 well plate and within each well contains a capillary known as the Cell®Safe. During centrifugal media evacuation the 3D culture is retained within the Cell®Safe and all liquid above the Cell®Safe is removed. The non-contact method of centrifugal media evacuation removes the risk of disrupting intricate cellular structures with an aspiration pin or pipette tip. The Blue®Washer can handle all SBS formatted plates, therefore increasing throughput to 384 and 1536 well plates is not a problem for dispensing and media evacuation. Blue®Plate will address two major difficulties in the 3D culture market - automation and standardization.

Clinical-grade preservation of Advanced Therapy Medicinal Products
Institute of Experimental Medicine of the CAS, Institute of Physiology of the CAS, BIOCEV
CZECH REPUBLIC

The biopreservation of cells and tissue-engineered grafts is one of the critical steps in the manufacturing of Advanced Therapy Medicinal Products (ATMPs), providing necessary time for quality control studies and/or ensuring the ready-to-use availability of the final product. In the presentation we will discuss the challenges occurred in clinical-grade preservation of cells and 3D grafts and share our experience in the development of cryopreservation and hypothermic storage strategies, applicable in ATMP manufacturing and further therapeutic application.

Development of a 3D cell model of skeletal muscle cells (myospheres) from human primary satellite cells.
University of Oslo
NORWAY

Nowadays, we know how muscle works in the body and how it can help us to counteract some diseases such as obesity. However, there is still a lack of information on how the muscle cells communicate with other organs in the body and how they respond or adapt to different situations at the molecular level. 2D cell models have been largely used to study energy metabolism concerning metabolic diseases but the result’s transferability to in vivo is limited. This project aimed to develop and characterize a human primary skeletal muscle 3D cell model (myospheres) as an easy, scaffold-free, and low-cost tool to later study impaired molecular mechanisms characteristic of metabolic disorders.

Animal-free and chemically defined coating solution for perfect 3D cell cultures
faCellitate GmbH
GERMANY

In vitro cell culture systems play a crucial role in biomedical research. In this context, the relevance of 3D in vitro model systems has increased because they have the potential to resemble in vivo microenvironment and human pharmacokinetics more reliably then 2D cell cultures. Our chemically defined, animal-free coating solution can be easily applied to cell culture surfaces creating a robust and homogeneous coating. Being biologically inert and preventing the unspecific binding of cells and proteins to several surfaces, BIOFLOATTM coatings represent an innovative platform for the generation of perfectly round shaped 3D spheroids in a reproducible and rapid way, outperforming current competitors. Importantly, they support the formation of single viable spheroids in microtiter multi well plates, being compatible with high-throughput assays.

AIDPATH - Modular Approach to AI-enabled CAR-T Cell Manufacturing
Fraunhofer Institute for Production Technology
GERMANY

CAR-T Manufacturing suffers from labor intensive manufacturing, biological variability of patient material while inflexible production protocols are employed. To improve the availability of CAR-T therapies to patient the AIDPATH Project aims to develop build and test a modular decentralized manufacturing system for CAR-T therapy. Automating us-ing a closed handling approach allows for parallelization while the modularity enables broad technological compatibility. Automated sampling for in-line quality control collects large amount of data on the cell samples which are fed in an AI-digital twin of the CAR-T product to support and adapt the manufacturing process. Automating the manufactur-ing process and accounting for biological variability is the key to optimize CAR-T cell production.

DATE, TIME & VENUE

  • Tuesday, 18 October 2022: from 10:00 to 18:00
  • Wednesday, 19 October 2022: from 10:00 to 17:00
  • WISTA Management Conventions. Rudower Chaussee 17, 12489 Berlin, Germany.

FEES & RULES
Academic Talks Free of charge for up to 25 minutes including Q&A
Product Demos Speakers will be charged 580,-€ (VAT excluded) for up to 30 minutes including Q&A

  • There is no submission fee.
  • Proposals must be submitted using the online form. Upon submitting a proposal, you will receive an automatic confirmation for your records if it was submitted successfully.
  • CellME Berlin reserves the sole right to accept or reject any proposal received without liability.
  • CellME Berlin does not pay for a speaking fee. Travel related expenses, meals and accommodations are the responsibility of the speaker.

SUBMIT YOUR PROPOSAL
SPEAKER BENEFITS

  • Complimentary full registration for the conference and exhibition.
  • Abstract book entry at no charge.
  • Free guest tickets to invite audiences.
  • Speaker Certificate in digital format.

LANGUAGE

Conference language is English.

Affiliated Online Meetings