| Day 1: Tuesday, 7 November 2023 | ||
| TIME | TOPIC | PRESENTER |
| 9:30 | Opening remarks | |
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| 17:00 | End | |
| Day 2: Wednesday, 8 November 2023 | ||
| TIME | TOPIC | PRESENTER |
| 9:30 | Opening remarks | |
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| 16:30 | End | |
Development of an organoid-based cell transformation assay for intestinal carcinogenicity screening of chemicals
Stony Brook Cancer Center, Stony Brook University, New York
United States
To identify potential intestinal carcinogens, we developed a cell transformation assay using mouse intestinal organoids (mASC-IOs) and assessed the transformation potential on 14 representative chemicals. We optimized the experiment protocol by determining the cytotoxicity, amplification and colony formation of chemical-treated mASC-IOs, and assessed its accuracy and in vivo relevance through characterizing tumorigenicity, pathology of subcutaneous tumor, and CRC-related molecular signatures. Remarkably, the carcinogenicity of 14 chemicals showed strong concordance with epidemiological findings and in vivo mouse studies. The quantitative analysis of anchorage-independent growth was significantly correlated with the tumorigenicity of tested chemicals in a dose-dependent manner. Importantly, the activity of chemical-transformed mASC-IOs was correlated with the differentiation status of subcutaneous tumors and the changes in oncogenic pathways associated with CRC development.
3-D Hollow Fiber Perfusion Bioreactors for Recapitulation of the In Vivo Microenvironment: A Better way to Grow Cells
FiberCell Systems Inc.
United States
Hollow fiber bioreactors provide a fundamentally more in vivo like way of culturing cells. They represent the only way to culture cells at in vivo like cell densities permitting the cells to generate their own specific microenvironment. More physiologic conditions result in uniform and complete post-translational modifications, reduced apoptosis and host cell protein contamination, and for complex cell-to-cell interactions to develop. Some cells will spontaneously form spheroids and others cells behave as they do in vivo. Specific examples include blood brain barrier, placenta co-culture, cryptosporidium culture and malaria sporozoite production for vaccines. Many techniques not possible by any other means can be performed in a 3-D hollow fiber bioreactor.
Single Use Disposable Inclined Settling Bioreactor for gentle expansion, concentration & harvest of cell and gene therapies
Sudhin Biopharma
USA
Inclined settling technology has been successfully demonstrated in biopharmaceutical manufacturing for achieving high cell densities and viabilities for extended culture durations. We have developed a novel compact scalable device with smaller footprint and higher inclined settling surface area and fabricated it as a single-use disposable plastic BioSettler for recycling of live and productive CHO cells back to the bioreactor, and continuous removal of dead cells and debris. We have now demonstrated it as an inclined settling bioreactor combining the features of a gentle air-lift cell culture bioreactor with the selective cell retention features of the inclined settler, making it useful for growing suspension cells like CHO, IPSC clusters, and attachment dependent cells like MSCs on microcarrier beads and HEK cells.
coming soon
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Modeling cardiac inflammation in engineered cardiac ventricular tissue reveals therapeutics for disease resolution
Goethe University Frankfurt
Germany
The talk will describe the generation of a novel developmentally-staged engineered iPSC-derived human ventricular cardiac tissue organoids comprising of all cell-types of native human cardiac ventricles with its concordant molecular, metabolic, structural and physiologic characteristics. These organoids exhibit the necessary vascularization and innervation to interrogate, at high resolution, the contribution of mutant immune cells on cardiac inflammation and consequent heart failure. In combining this model with synthetic lethality screening, we identify effective inhibitors of inflammation and cardiopathology. Given the high unmet therapeutic need, our data suggests potential benefit of these compounds for clinical translation.
New non-invasive, label-free monitoring approach for 2D and 3D cell culture
Ludwig-Maximilians-University Munich, PHIO scientific GmbH
Germany
We developed a novel lensfree imaging method exploiting the optical properties of the cell itself for imaging inside the incubator, which allows non-invasive, super compact, lable-free, live-cell monitoring. By applying AI to determine key cell culture parameters such as confluence, proliferation, and cell motility, high-quality, automated, objective, and real-time data can be collected. Applying our lensfree microscopy (LM) method, we find that memory effects from heterogeneous cell culture conditions lead to an increase of variance during subsequent assays like e.g. omics-readouts or other cell based assays, like wound healing assays, motility and proliferation assays significantly. Furthermore, our LM is also suitable for 3D applications and will enable quantification of organoid growth dynamics and interactions.
From Microenvironments to Automation: Supporting Reproducibility of Cell Manufacturing and Organoid Research
Oxford Optronix Ltd.
United Kingdom
For over two decades, Oxford Optronix has been dedicated to ensuring reproducibility in cellular research. Here we discuss two widely utilized systems that contribute to this mission in both cell manufacturing and organoid research. Firstly, the GelCount, an automated imaging and analysis platform for colony, spheroid, and organoid counting/sizing is presented. This automation not only saves time and eradicates human bias, but also greatly improves the reproducibility of all counts across a study. Secondly, the HypoxyLab incubator and workstation is introduced with its unique approach for setting a precise oxygen concentration environment independent of altitude or atmospheric pressure changes - a critical factor affecting reproducibility of every hypoxia/physoxia based study.
coming soon
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Agrostemmoside E: a potent tool for transfection assays
Freie Universität Berlin
Germany
Transfection is the process to bring nucleic acids into eukaryotic cells. The application of nanoparticles is a standard way to achieve this goal. However, once they reach the cellular membrane, they are endocytosed and trapped in early endosomes, which are acidified to become lysosomes. This process leads to degradation, and it is established that only one percent of the genetic material is able to escape the endosome into the cytosol, making this step the major current obstacle for efficient delivery of the genetic cargo. Agrostemmoside E, a plant secondary metabolite, triggers endosomal escape and application of this molecule in cellular assays can reduce genetic material needed for any transfection assay, since more genetic material reaches the cytosol.
Vital Light 3D – Towards Next Breakthrough in Bioprinting
Vital3D Technologies
Lithuania
The rapid advancement of 3D bioprinting opens new opportunities and creates ever-complex challenges. One of them is the capability to 3D print biostructures with high throughput and small feature sizes. This is necessary for such applications as lab-on-chip or printing organs with embedded functioning vasculature. In this talk we present a 3D bioprinter ‘’Vital Light 3D’’ designed to meet this challenge. Based on the femtosecond 2-photon polymerization technique paired with spatial beam shaping it is capable of printing cm-sized objects in a few hours while maintaining µm level precision. We discuss these capabilities in detail explaining how they are achieved, showing already printed sample structures, and highlighting how this printing technology can transform the 3D bioprinting field in the future.
Novel automated picodroplet-based technology to streamline cell line development and cell engineering workflows
Sphere Fluidics Ltd.
United Kingdom
Generation of a highly expressing, regulatory acceptable clonal cell line is the first key step in the development of a biopharmaceutical. To provide the key characteristics (Productivity, Stability, Quality, Monoclonality, and Scalability) from a cell line is challenging especially when using traditional techniques. Significant bottlenecks develop when screening for these rare cells and lengthen the cell line development workflows. Similarly, cell engineering strategies for biotherapeutics discovery frequently add costs and time. Our Cyto-Mine® platform, powered by picodroplet-based microfluidic technology, streamlines the cell line development workflow. It offers a powerful solution to the challenges of screening large numbers of cells allowing isolation of the highest value clones while ensuring monoclonality and enables a viable high-throughput cell engineering workflow.
coming soon
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The conference is conducted in person. There is no virtual or hybrid option for attendees.
This is an onsite event only. The event will not be recorded as the speakers might share their unpublished results.
The conference language is English.
BioCHIP Berlin - International Forum on BioChips and BioChip Solutions